Kazakhstan journal for oil & gas industry

Background: Today, the oil industry is one of the main sources of environmental problems in Western Kazakhstan. Therefore, cleaning soils from oil contamination becomes extremely important.

Aim: The study is aimed at searching and isolating highly effective hydrocarbon-oxidizing bacteria, which are native to highly mineralized soils of the Uzen oil field.

Materials and methods: The work uses microbiological methods for cultivating bacteria, as well as chemical (composition of water) and analytical (IR spectrometry) methods.

Results: 4 enrichment cultures of hydrocarbon-oxidizing bacteria were obtained, 3 pure cultures of oil-oxidizing bacteria were isolated. Their hydrocarbon-oxidizing efficiency has been studied.

Conclusion: In this work, active enrichment cultures of halophilic and moderately thermophilic hydrocarbons were obtained, characterized by high biotechnological potential, capable of oxidizing a wide range of hydrocarbons, including high-molecular polycyclic and sulfur-containing compounds, as well as new strains of halophilic hydrocarbon-oxidizing bacteria, the potential of which will be studied in further work. 

Aim   Under conditions of anthropogenic impact, the chemical composition of water in surface rivers and groundwater bodies is subjected to pollution, which results in not only a decrease in water quality, but also an increase in the number of pathogenic and conditionally pathogenic bacteria. This work analyzes the chemical and microbiological composition of natural water, as well as recycled water.  The purpose of this work is to study physicochemical and microbiological parameters of natural, industrial recycled water and treatment with new generation coagulants based on activated aluminum alloys with the content of metal-activators from 0.5 to 1.0 wt%.

Matherials and Methods As natural waters were analyzed: natural waters from water intake "Almaty SU", tract "Medeu", from the well of pilot metallurgical production (PMP) of the Institute of Metallurgy and Ore Beneficiation (IMOB), the river Zhaiyk (Ural). As recycled water was analyzed water sampled from the water treatment unit of deep oil refining production (WTU of PDOR)

Aluminum polyoxychlorides (PAC) were obtained by interaction of aluminum activated by metal-activators (indium, gallium, tin) in the amount of 0.5-1.0 weight % each with 3% hydrochloric acid.  Quality parameters - mass fraction of AI in terms of Al2O3 33,2-36,9%, basicity 56-67%, corresponded to GB 15892-2009 standard.

Water samples were taken according to state standard No59024-2020, were evaluated by organoleptic (smell, taste, color), physical (turbidity, pH, conductivity, permanganate oxidation), chemical parameters (total salt content, the content of anions - hydrocarbonates, sulfates, chlorides) in accordance with the sanitary rules, approved by Order of the Minister of Health Republic of Kazakhstan dated February 20, 2023 № 26 and ISO5667.

Water treatment methodology: a certain amount of PAC was injected into 500 ml of test water while intensely stirring, after 3 minutes the speed was reduced to 50 rpm for 15 minutes, and was allowed to stand for 30 minutes afterwards.

Permanganate index (PI) was determined according to ISO 8467:1993.

Turbidity was measured directly using a HACH 2100Q Turbidimeter (EPA)and 2100Qis Turbidimeter (USA). Cell morphology of microorganism cultures was studied by light microscopy using a MicroOptix MX-1150 (T) stereoscopic-соtrinocular microscope (magnification 1250)  

Conclusion An effective and technologically simple method for producing aluminum polyoxychloride (PAC) was developed, which involves dissolving activated aluminum alloy containing 1.5 to 3.0 wt.% of an activating additive (indium, gallium, tin) in 3% hydrochloric acid.

Changing the alloy composition and reaction conditions makes it possible to adjust the properties of РAC (мass fraction of aluminum in terms of Al₂O₃ is from 33.2 to 41.0%, basicity - from 55.1 to 66.5%). Natural and recycled waters were treated with synthesized PAC to the requirements established by the regulatory documents for drinking water and recycled water. The investigated parameters included turbidity, permanganate index, microbiological composition in samples treated with PAC. The effectiveness of reducing turbidity in domestic and drinking water reaches 90 - 99%. The permanganate index of natural water is reduced by 93%. Synthesized PAC exhibits bactericidal properties tested on water sampled from Medeu tract.

Introduction. Nowadays there are few works in the country on obtaining targeted products from bituminous sands. Among them, scientific works on the method of separation using ultrasound irradiation can be classified as non-traditional technologies. This method has many advantages, in particular energy and water saving, high production in a short time and allows to create a mobile unit installation. Determining the necessary frequency and power depending on the research object of ultrasound technology are the main process parameters. The first scientific studies on the extraction of bitumen from bituminous sands using ultrasound have been carried out since the 1990s. The results of the work showed that in an alkaline solution ultrasonic cavitation of bituminous sands, the degree of bitumen extraction increases. The purpose of the work. To fill the deficit of bitumen in Kazakhstan, study the influence of ultrasonic technology on the extraction of bitumen from bituminous sands and determine the physical and chemical characteristics of the obtaining product. Research results. A distinctive feature of the bituminous sands chosen as the object of study is that the bituminous sands come to the surface and are located in open areas. Long-term exposure to the atmosphere led to the loss of light fractions, and the heavy components of bitumen were subjected to oxidation processes under the influence of various technogenic factors. These processes led to the formation of heavy oils and hard bitumens of complex composition. Visual analysis of a bituminous sands sample shows a rich black color with a bituminous sheen. As a result of the study of the influence of ultrasonic technology on the extraction of bitumen from bituminous sands the physical-chemical characteristics of obtained bitumen were studied. During the testing of this technology, the optimal parameters of the process were determined depending on the object of research. According to the results of the study, it was found that the clay compounds present in the bituminous sands have a direct impact on the process of determining the power of ultrasound. Conclusion. Ultrasound technology has been shown to be a promising and industrially applicable technology for extracting bitumen from bituminous sands.

Additional study of field structures to identify new areas and determine the oil potential by assessing the prospects of the marginal parts of the fields identified by VRS MOGT-3D. The expected results are important in increasing hydrocarbon potential. Oil and gas prospects are associated with fairly well studied and developed Jurassic deposits in Southern Mangyshlak, the potential of which is far from being exhausted, as evidenced by underexplored uplifts.