Қазақстанның мұнай-газ саласының хабаршысыҚазақстанның мұнай-газ саласының хабаршысы2707-42262957-806XKMG Engineering10890010.54859/kjogi108900Study of the sulfidogenic bacteria activity of the field Kazakhstan formation microflora and its potential contribution to corrosion processesBissenovaMiua Allaberdyevna<p>PhD,<strong> </strong>Lead Engineer, Field Chemistry Laboratory</p>Miua@mail.ruhttps://orcid.org/0000-0002-9117-0931BidzhievaSalimat Khasanovna<p data-start="52" data-end="105">Degree: Candidate of Biological Sciences, Position: Research Scientist, Subdivision: petroleum microbiology laboratory</p>
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<p> </p>salima.bidjieva@gmail.comhttps://orcid.org/0000-0002-7599-114XBranch of KMG Engineering LLP KazNIPImunaigazS.N. Winogradsky Institute of Microbiology, Federal Research Center for Biotechnology, Russian Academy of Sciences812507202516022026Copyright © , Bissenova M.A., Bidzhieva S.K.<p><strong>ABSTRACT</strong></p>
<p><strong>Background. </strong>Today at the oil field «N» there is an intensification of corrosion complications. Repeated monitoring of corrosion in all sections of the field has shown the localization of aggressive gases in section B, where thermal oil displacement technologies are used, which could lead to increased corrosion processes. In addition, extensive studies of the microbial community of aquifers were carried out at the field, which revealed the presence of sulphate recovery agents as well as wandering sulfides bacteria that could also contribute to the development of an aggressive environment. However, the influence of the microbial community on corrosion development remained an important issue and studies were carried out to identify the contribution of sulfidogenic bacteria to the formation of corrosive environments in this field.</p>
<p><strong>Aim.</strong> To study the contribution of sulfidogenic bacteria of production waters to the processes of an aggressive environment formation and corrosion in the field.</p>
<p><strong>Materials and methods. </strong>The objects of study were samples of water derived from group installation (GI), waste water units of cluster pumping facility (CPF) and vertical steel reservoirs (VSR).</p>
<p>The studies were carried out using classical microbiological methods: anaerobic cultivation of bacteria, seeding by a method of ten-fold reproductions, obtaining biomanufacturing of the studied object in a natural environment, etc. in order to determine the number of sulphidogenic bacteria in plankton and adsorbed constituents in microflora of commercial waters, as well as their effect on corrosion of metal witness samples.</p>
<p>To determine the content of hydrogen sulfide and carbon dioxide applied titrimetric method. Oxygen concentration was established by a rapid method using the Fibox 4 fiber-optic oxygen analyzer by PreSens. The amount of mechanical impurities was determined through filtration followed by gravimetric analysis. The corrosion rate of steel samples-witnesses were investigated using the gravimetric method with a U-shaped cell.</p>
<p><strong>Results</strong>. The number of planktonic and adhesive bacteria at the objects has been determined, the productivity of different physiological groups of sulfidogenic bacteria has been shown, the corrosion rate of samples has been investigated metal samples under the influence of adhesive bacteria in combination with other related factors.</p>
<p><strong>Conclusion</strong>. The microflora of the deposit’s working waters has a high corrosion potential, but the greatest contribution to the formation of an aggressive environment is due to the application of thermal oil-recovery technologies in the deposit.</p>hydrogen sulfidecarbon dioxidesulfate-reducing bacteriasulfidogenic bacteriacorrosion rateaggressive gases.күкіртті сутеккөмірқышқыл газсульфатты қалпына келтіруші бактерияларсульфидогенді бактерияларкоррозия жылдамдығыагрессивті газдар.сероводородуглекислый газсульфатвосстанавливающие бактериисульфидогенные бактериискорость коррозииагрессивные газы.[1. Елеманов Б.Д., Герштанский О.С. Мұнай өндіру кезіндегі қиындықтар. – М.: Наука, 2007. – 420 б.][2. Дияна С. Соколова, Екатерина М. Семенова, Денис С. Груздев, Салимат К. Биджиева және басқалар. Қазақстандағы жоғары температуралы Узень мұнай кен орнындағы сульфидогенді микробты қауымдастықтар // Microorganisms. — 2021. Т. 9, № 1818. https://doi.org/10.3390/microorganisms9091818][3. Пуэнтес-Кала Э., Тапиа-Пердомо В., Эспиноса-Вальбуэна Д., Рейес-Рейес М., Кинтеро-Сантандер Д., Васкес-Даллос С., Салазар Х., Санта-Мария-Гальвис П., Сильва-Родригес Р., Кастильо-Вильямисар Г. Микроорганизмдермен индукцияланған коррозия: зерттеу саласындағы олқылықтар // Frontiers in Environmental Science, Т. 10, 2022. https://doi.org/10.3389/fenvs.2022.924842][4. Кевбрин В.В., Заварзин Г.А. Күкірт қосылыстарының Acetohalobium arabaticum галофильді гомоацетатты бактериясының өсуіне әсері // Микробиология. — 1992. — Т. 61. — Б. 812–817.][5. Виддель Ф.Ф., Бак Р. Грамтеріс мезофильді сульфатты тотықсыздандыратын бактериялар // Прокариоттар: Бактериялар биологиясы жөніндегі анықтамалық: Экофизиология, изоляция, идентификация, қолдану / ред. Баловс А. және басқалар. Берлин: Springer, 1992. Б. 3352–3378.]